PCR
PCR / PCR. [^cronim engi. pentru Poiymerase Chain Reaction = reacfia w [anf a pohmerazei] Metoda de amplifi-care electiva in vitro a secventelor de acizi nucleici. ProcedeuI este revolutionar, deoarece pemnite obtinerea, fara *clonare, a unei amplificari considerabile a unei tinte date de ADN, ded un castig enorm In sensibilitate. Astfel, daca metoda clasica (*Southern blot) reclama cel putin 1 mg de ADN genomic, adica echivalentui a 150 000 celule (admitand un randament de extractie maxim de 100%), se pare ca limita inferioara de sensibilitate a metodei PCR poate cobori la o singura celula. Ca urmare, metoda este utila In mod particular pentru detectia secventelor foarte putin reprezentate Intr-un esantion biologic (ex.: don celular leucemic rezidual, particula virala). Sin.: ampli-ficare prin reactie In lant a polimerazei, reactie In lant a polimerazei.patient contact record; perinatal clinical record; phosphocreatinine; photoconvulsive response; plasma clearance rate; polymerase chain reaction; post-compression remodeling; prehospital care report; principal component regression; protein catabolism rate
phosphocreatine
plasma creatinine
protein catabolic rate
patient contact record; perinatal clinical record; phosphocreatinine; photoconvulsive response; plasma clearance rate; polymerase chain reaction; post-compression remodeling; prehospital care report; principal component regression; protein catabolism rate
phosphocreatine
plasma creatinine
protein catabolic rate